2014/2015
miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells
International Journal of Oncology*, 2015,46(3):1169-1180
- IFR: Top 45.5% in Oncology
Author(s) | Bin Fu
Yibing Wang Xiali Zhang Bin Lang Xiaocheng Zhou Xiaoyuan Xu Tao Zeng Weipeng Liu Xu Zhang Ju Guo Gongxian Wang |
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Summary | Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR‑221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR‑221. As a result, the repression of miR‑221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells. |
* With an impact factor among those of the top 45.5% of journals in Oncology